ZFIN Image: Liu et al., 2023, Figure 4

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Figure Caption

Figure 4

PGE2–EP4 signaling promotes the proliferation of renal progenitor cells (RPCs) during nephron regeneration.

Tg(lhx1a:DsRed) fish were injected with EdU to label proliferating nuclei at 5 dpi and kidneys were harvested 3 hr later. (A, B) Gentamicin induced lhx1a⁺ cell aggregates with proliferating EdU⁺ nuclei. (C, D) The proliferation of lhx1a⁺ cell aggregates in cox2a^-/- was significantly less than that in WT. (E, F) Intraperitoneal injection of dmPGE2 could promote the proliferation of lhx1a⁺ cells in cox2a^-/-. (G, H) GW627368X inhibited the proliferation of lhx1a⁺ cells. (I, J) Injection of dmPGE2 could not rescue the GW627368X treatment. n = 5–7 in each condition. Scale bar in (A, C, E, G, I), 50 μm; (B, D, F, H, J), 100 μm. The images on the right showed a higher-magnification image (arrowheads). (K) Proliferation ratio of lhx1a⁺ RPCs in (A–J) was calculated using ImageJ. n = 5–7 in each condition. Data were analyzed by ANOVA, ***p<0.001; ns, no significant difference.

Acknowledgments

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