ZFIN Image: Fung et al., 2023, Figure 5

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Figure 5

Expression of ZAAT in HepG2 cells suppresses SREBP2 target genes. (A) Transgenes used for HepG2 transfection (top panel). Immunoblot of HepG2 cell lysates for eGFP as an indication of transgene expression levels using β-tubulin as a loading control (bottom panel). qPCR analysis was performed from cDNA generated from HepG2 cells with stable overexpression of (B) eGFP-T2A-ZAAT and (C) eGFP-T2A-AAT. Expression values were normalised to the housekeeping genes GAPDH, ACTB and TBP. An unpaired student’s t-test was used to determine the statistical significance for each gene. Error bars indicate mean ± s.e.m. * p < 0.05, *** p < 0.001.

Acknowledgments

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