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Fig. 5 crk and crkl are necessary for proper embryonic development and proper eye size in zebrafish. A, Brightfield whole mount images of 72 hpf embryos resulting from crk heterozygous/crkl heterozygous (CcLl) incross. Genotype of each representative embryo is shown on bottom left corner of the image: C, wild-type crk allele; c, mutant crk allele; L, wild-type crkl allele; l, mutant crkl allele. Bottom right corner of each image shows the actual percentage (red) over expected percentage (black) of embryos of that genotype randomly selected and successfully genotyped from 230 embryos (N = 9). Red star above cc genotype actual percentages denotes unequal proportions. Scale bar at 250 μm in CCLL image. B, Graph of eye diameter; C, body length; and D eye diameter normalized over body length for each genotype analyzed by one-way ANOVA and Tukey multiple comparison test (eye diameter ANOVA P = .000043, CCLL vs. ccll P = .00022; body length ANOVA P = .000005, CCLL vs. ccll P = .000022; eye diameter normalized to body length ANOVA P = .00962, CCLL vs. ccll P = .0307; N = 10, n = 230)