ZFIN Image: Morgan et al., 2023, Figure 6

Image

Figure Caption

Figure 6

ahctf1 heterozygosity restricts DNA replication in dox-treated TO(kras^G12V)^T/+ hepatocytes in the presence and absence of Tp53.

(a) Representative maximum intensity projection images of EdU incorporation (white puncta) into TO(kras^G12V)^T/+ livers of the indicated ahctf1 and tp53 genotypes. Scale bar 25 µm. (b) Quantification of the percentage of EdU-positive nuclei per Hoechst 33342-positive nuclei (n ≥ 25). (c) Impact of ahctf1 heterozygosity and homozygous tp53 mutation on liver volume in dox-treated TO(kras^G12V)^T/+ larvae (n ≥ 17). (d) RT-quantitative PCR (RT-qPCR) analysis of mRNA expression of the cell cycle regulators, cdkn1a and cdkn2a/b and (e) tp63 and tp73 expression in TO(kras^G12V)^T/+ micro-dissected livers of the indicated ahctf1 and tp53 genotypes (n = 3 biological replicates). Data are expressed as mean ± standard error of the mean (SEM). Significance was assessed using a one-way analysis of variation (ANOVA) with Tukey’s multiple comparisons test.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife

Figure 6

Figure 6

ahctf1 heterozygosity restricts DNA replication in dox-treated TO(krasG12V)T/+ hepatocytes in the presence and absence of Tp53.

ahctf1 heterozygosity restricts DNA replication in dox-treated TO(kras^G12V)^T/+ hepatocytes in the presence and absence of Tp53.