ZFIN Image: Morgan et al., 2023, Figure 3

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Figure Caption

Figure 3

ahctf1 heterozygosity impairs mitotic spindle assembly and chromosome segregation in dox-treated TO(kras^G12V)^T/+ hepatocytes.

(a) Representative Airyscan imaging of liver cryosections stained with α-tubulin antibody (magenta) marking spindle microtubules and DAPI (cyan) marking DNA in mitotic cells of TO(kras^G12V)^T/+ larvae on a wildtype ahctf1^+/+ background. (b) Mitotic cells in liver cryosections of TO(kras^G12V)^T/+ larvae that are heterozygous for ahctf1^+/− exhibit multiple defects, including multipolar spindles, misaligned chromosomes, and anaphase bridges (arrows). Scale bar 2 µm. (c) Distribution of cells observed at different mitotic stages (n = 92 livers, 326 mitotic cells). (d) Quantification of the percentage of mitotic hepatocytes exhibiting an aberrant phenotype (n = 14–57). Significance was assessed using a Chi-square test.

Figure Data

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Acknowledgments

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Figure 3

Figure 3

ahctf1 heterozygosity impairs mitotic spindle assembly and chromosome segregation in dox-treated TO(krasG12V)T/+ hepatocytes.

ahctf1 heterozygosity impairs mitotic spindle assembly and chromosome segregation in dox-treated TO(kras^G12V)^T/+ hepatocytes.