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Fig. 6
Follistatin (FST) negatively controls activin signaling and predicts the incidence of acute-on-chronic liver failure (ACLF). (A) Concentrations of activin and FST were measured by ELISA in 10 patients with MHL-LF and 13 healthy volunteers. The ratio of activin and FST was calculated. (B) Immunohistochemistry for p-SMAD2 is shown in 2 representative patients with MHL-LF. (C) Positive number of phosphorylated SMAD2 (p-SMAD2) in 10 patients with MHL-LF was counted. (D) Kaplan-Meier curves analysis was performed to calculate the incidence of ACLF in 186 patients with cirrhosis, as described in the Materials and Methods
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