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Figure 5
Effect of G-CSFR truncation mutation on emergency hematopoiesis. Wild-type (wt/wt) and homozygous (mdu27/mdu27) mutant csf3r embryos, either uninjected or injected with mRNA encoding G-CSF (+ csf3a) were subjected to WISH with mpo during primitive (A–D) and early definitive (F–I) hematopoiesis, with representative images shown. Individual embryos were assessed for the number of mpo+ cells for primitive (E) and definitive (J) hematopoiesis, with mean and SEM shown in red and level of statistical significance indicated (***: p < 0.001, *: p < 0.05, ns: not significant; n=17-30).
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