ZFIN Image: do Amaral et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Desmin accumulates at the perinuclear region of early somites in zebrafish embryos. 24-h zebrafish embryos were labeled with an antibody against the muscle-specific intermediate filament protein desmin (red, in A–K) and with the nuclear dye DAPI (cyan, in A–K). Higher magnifications of the area marked in the inset in (A) are shown in images (B–K). Immunofluorescence confocal images of different focal planes (1 μm apart, in B–K) show the perinuclear localization of desmin (white arrow in D) in somite 28 at the most caudal region of a 24-h zebrafish embryo. Desmin is also found at the septa between adjacent muscle somites 28 and 29 in zebrafish embryos (black arrow in K). Scale bar in K = 10 μm. N = 4 independent experiments.

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Reprinted from Biochimica et biophysica acta. Molecular cell research, 1869, do Amaral, M.J., de Andrade Rosa, I., Andrade, S.A., Fang, X., Andrade, L.R., Costa, M.L., Mermelstein, C., The perinuclear region concentrates disordered proteins with predicted phase separation distributed in a 3D network of cytoskeletal filaments and organelles, 119161, Copyright (2022) with permission from Elsevier. Full text @ BBA Molecular Cell Research