ZFIN Image: Brandt et al., 2021, Fig. 8

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Fig. 8

Macrophage ablation alters scleral accessibility to a collagen hybridizing peptide. (A–C) Representative images of central retina sections from mfrp⁺^/^–; mpeg1:NTR-eYFP⁺ and mfrp^–/^–; mpeg1:NTR-eYFP⁺ MTZ treated and untreated fish. (A–A''') Grayscale CHP images. (B–B''') Colorized and merged images with DAPI in cyan and CHP in red. (C–C''') High-magnification images of regions of interest in the posterior sclera as indicated by white outlines in (B) to (B'''). (D, E) Quantification of the mean pixel intensity of the CHP staining across full sclera (D) and at central posterior sclera (E). For whole sclera (D), both genotype (P = 0.001) and MTZ treatment (P = 0.022) had a significant effects. In the posterior sclera (E) only genotype (P = 0.0204) had a significant effect. Two-way ANOVA was used for statistical analysis. P values are shown from Sidak's multiple comparisons for post hoc analysis. Error bars represent standard deviations. *P < 0.05. For mfrp⁺^/^– untreated, n = 6; for mfrp⁺^/^– MTZ treated, n = 6; for mfrp^–/^– untreated, n = 6; for mfrp^–/^– MTZ treated, n = 5.

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Acknowledgments

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