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FIGURE 7
Smc5 is required for 3T3‐L1 adipogenesis. (A) The 3T3‐L1 white preadipocytes were infected with lentivirus shRNAs targeting Smc5 (Smc5‐shRNA) or scramble (Scr‐shRNA) virus, followed by adipogenesis assay. Quantitative real‐time PCR (qPCR) and Western blot confirmation of Smc5 knockdown efficiency before adipogenesis. Values represent the mean ± s.d. (B) Oil red O staining at D7 of adipogenesis. Scale bars = 30 μm. (C) Western blots of PPARγ, C/EBPα and GLUT4 in preadipocytes (D0) and adipocytes (D7). (D) qPCR of Pparg, Cebpa, Fabp4, Adipoq, Slc2a4 expression at D0 and D5 of adipogenesis. Values represent the mean ± s.e.m. (E) Western blots of phosphorylated Akt at Serine 473 and total Akt in protein extracts of adipocytes (D7). (F) Insulin‐stimulated glucose uptake assessed by the 2‐deoxyglucose assay in adipocytes (D7). Values represent the mean ± s.d. In parts (A and D), Rplp0 (36B4) was chosen as the internal reference gene. For parts (A, D and F), the