Fig. 6

Fig. 6

Fig. 6. Inhibition of Wnt/?-catenin signaling prevents cardiomyocyte dedifferentation. (A) embMHC and MF20 immunostaining on cryoinjured hearts of hs:Axin1-YFP fish and wild-type siblings, heat-shocked daily from 1 to 7 dpi. n (hearts) ?= ?7 (wild-type), 8 (hs:Axin1-YFP). In the magnified view, the cyan dotted lines represent the outline of the MF20⁺ ?cardiomyocyte area. Scale bars, 100 ??m (overview), 10 ??m (magnification). (B) Quantification of the embMHC⁺MF20⁺/MF20⁺ area in the 50 ??m wound borderzone of images shown in (A). Error bars represent CI 95%. Unpaired Welch's unequal variances t-test. (C) MF20 and GFP immunostaining on cryoinjured hearts of ?14.8gata4:GFP fish treated with DMSO or IWR1 from 1 to 7 dpi. n ?= ?8 (DMSO), 6 (IWR1). Scale bars, 100 ??m (overview), 10 ??m (magnification). (D) Quantification of GFP⁺MF20⁺/MF20⁺ area in the 50 ??m wound borderzone of images shown in (C). Error bars represent CI 95%. Unpaired Welch's unequal variances t-test.