ZFIN Image: Elsaid et al., 2022, Figure 4

Image

Figure Caption

Figure 4

Immunohistochemical and Western blot detection of selected proteins in kidneys from WT and M ZF reveals protein expression disturbances in mitochondria and lysosomes. (A) Representative IHC staining specific for mitochondrial marker isocitrate dehydrogenase (NAD⁽⁺⁾)3 alpha (Idh3a) and lysosomal marker cathepsin B (Ctsb) in kidney tissue sections from WT and M ZF (upper right and upper left panels, and lower right and lower left panels, respectively; (B) Quantification of immunohistochemical staining of sections from WT and M ZF kidneys. Signal intensity is significantly higher in WT than in M for both proteins. (C) Representative immunoblots of Ctsb and Idh3a in kidney tissue section from WT and M ZF; (D) Quantification of immunoblots from WT and M ZF kidneys. (Mann–Whitney test U * p < 0.05). Wildtype (WT), mutant (M). ns: not significant. Scale bar (bottom left corner, in black) = 100 µm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.