ZFIN Image: Chen et al., 2022, Fig 9

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Figure Caption

Fig 9

Expression of fshr, cyp19a1a and activins (inhbaa, inhbab and inhbb) in PG follicles from single (gdf9-/- and inha-/-) and double mutant (gdf9-/-;inha-/-).

(A-E) The PG follicles were isolated from the fish of different genotypes at 43 dpf, shortly before puberty onset or PG-PV transition occurs in the control fish (normally around 45 dpf). The expression of inhbaa was significantly reduced in gdf9-/- mutant; however, it was reversed by inha mutation in the double mutant together with fshr and cyp19a1a, but not inhbab and inhbb. Different letters indicate statistical significance (P < 0.05, n = 3 biological replicates). (F) Stimulation of Smad2/3 phosphorylation (p-Smad2/3) in the PV oocytes by activin in vitro. The PV follicles were isolated and treated with recombinant goldfish activin B (5 U/ml) for 2 h followed by immunofluorescent staining for p-Smad2/3.

Acknowledgments

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