Figure 8

Mutations in her6 enhance the jag1b mutant phenotype, her6 and her9 are redundantly required for eye development. (A) Exon diagram for her6 indicating the untranslated regions (UTR) and the regions predicted to encode the basic helix-loop-helix (bHLH) and Orange domains. The location of the deletion encoded by the her6^co3005 allele is indicated. (B) jag1b;her6 double heterozygotes were intercrossed and six days post fertilization (dpf) larvae were stained with Alcian Blue and Alizarin Red to label cartilage and bone. The individuals were then genotyped, the viscerocrania were dissected and flat mounted then imaged. The following craniofacial skeletal elements are indicated in the wild-type individual: opercle bone (op), branchiostegal ray (br), cleithrum (cl), foramen (f). The dysmorphic foramen phenotype (df) is indicated, and the small op bone phenotype is indicated by an arrowhead. Scale bar is 50 μm (C) Genotyped preps from B were scored for penetrance of jag1b mutant-associated phenotypes. Asterisk in small op bone row indicates that penetrance in jag1b-/-;her6-/- is significantly different from jag1b-/-;her6+/+ (p<0.05). (D) her6;her9 double heterozygotes were intercrossed and six days post fertilization (dpf) larvae were stained with Alcian Blue and Alizarin Red to label cartilage and bone. The individuals were then genotyped and whole-mount imaged. An arrow indicates unmineralized opercle. The lens (l) is indicated in the wild type and a double lens phenotype is indicated in double homozygous mutants (dl).