ZFIN Image: Lee et al., 2021, Fig. 3

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Figure Caption

Fig. 3

Comparison between 3D immunostaining of tissue cleared with SDS and SDS?OPTIClear. (A) Iba?1 staining of tissues cleared with 4% SDS?OPTIClear (left panel) and 4% SDS only (right panel). Upper panel: images taken with a 10� objective. Lower panel: zoomed?in image of the dentate gyrus (DG) taken with a 20� objective (left image), Z?stack images taken with a 63� objective with z?depth = 161.7 ?m (right image). (B) TOMM?20 staining of regions cleared with 4% SDS?OPTIClear (left panel) and 4% SDS only (right panel). Left image: 20� images of DG regions. Right image: z?stack of the images showed on the left (z?depth = 125.84 ?m). (C) ChAT staining taken with a 20� objective. Left panel: 20� images of the nbM cleared with 4% SDS and 4% SDS?OPTIClear. Right panel: z?stack of the images showed in the left panel (z?depth = 72 ?m). The same imaging and display settings were used for both 4% SDS and 4% SDS with OPTIClear conditions

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuropathol. Appl. Neurobiol.