ZFIN Image: Liu et al., 2022, Fig 3

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Fig 3

Knock-down of candidate genes disturbed heart looping.

(A) Different types of zebrafish heart are shown in Tg (cmlc2: eCherry) morphants in ventral view: dextral loop (normal), sinistral loop (abnormal), and no-loop (abnormal). (B) The percentage of abnormal heart looping with morphants injected. Bars show the total percent of abnormally looped hearts including two types: no-loop and sinistral loop hearts. (C) The corresponding mRNA can rescue LR randomization caused by MO of candidate genes. (D) The ratio of the abnormal heart looping of embryos generated by co-injection of zebrafish Cas9 mRNA and gRNA. Each experiment was repeated at least 3 times with > 50 embryos examined for each group every time. Standard MO (Std) is used as the negative control. galnt11MO is used as the positive control. Chi-squared test (continuity corrected) was used in B, C and D; *P <0.05, **P < 0.01, ***P < 0.001, respectively vs. Std-16ng (glant11, trip11, dnhd1, and cfap74) or Std-2ng (egr4). WT, wild type.

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Acknowledgments

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