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Figure 9
Zebrafish smyd3 augments hypoxia signaling. A, alignment of smyd3 amino acid sequences from human, mouse, and zebrafish, and the consensus sequence is shown below. B–D, qPCR analysis of pdk1 (B), vegf (C), and phd3 (D) mRNA in ZFL cells transfected with or without pCMV-smyd3 and cultured under normoxia (21% O2) or hypoxia (1% O2) for 24 h. EV, pCMV empty vector (control). Data show mean ± SD; Student’s two-tailed t test. ∗p < 0.05, ∗∗p < 0.01. Data from three independent experiments. qPCR, quantitative RT–PCR; ZFL, zebrafish liver.
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Full text @ J. Biol. Chem.