ZFIN Image: Chrispell et al., 2022, Figure 1

Image

Figure Caption

Figure 1

Immunospecificity of antibodies against phosphorylated Grk1 and Grk7.A, FLAG-tagged, purified recombinant zebrafish Grk1a and Grk1b treated in vitro with either λ phosphatase or PKA⍺ catalytic subunit were subjected to immunoblot analysis. Immunoblots were probed with antibodies against the FLAG-tag (green) and phosphorylated Grk1 (red) followed by incubation with secondary antibodies. B, Adult zebrafish were light- or dark-adapted, euthanized, and retinal homogenates subjected to immunoblot analysis. Immunoblots were probed with antibodies against Grk1 (green) and phosphorylated Grk1 (red), followed by incubation with secondary antibodies. C, Larvae at 5 dpf were light- or dark- adapted, followed by incubation with forskolin or vehicle (dimethyl sulfoxide [DMSO]) for 30 min. Larvae were euthanized and intact eyes were harvested for immunoblot analysis. Immunoblots were probed against Grk7 (green) and phosphorylated Grk7 (red) with anti-Grk7 antibody directly conjugated to CF770 and anti-phosphorylated Grk7 antibody directly conjugated to CF680. Panel C was spliced for clarity due to noncontiguous loading on the same gel.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.