ZFIN Image: Lee et al., 2022, Figure 2

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Figure Caption

Figure 2

The cellular structure of whole-mount retinal vessels isolated from adult transgenic zebrafish, Tg[fli1:EGFP]. The vessel-specific expression of GFP (A) and nuclear staining with DAPI (B) in the retinal vasculature. (C) Merged image of (A,B). Retinal vessels from the optic disc (OD) branched out into the arterial region (AR) and then the capillary area (CA). Asterisks indicate membrane regions between blood vessels. (D–I) Immunofluorescent labeling of tight junction protein (ZO-1, red), nuclear staining (Hoechst, blue), and GFP expression (green) in different retinal vessel regions; arterial region (D–F) and capillary area (G–I). Endothelial cells are elongated and their long axis is parallel to the line of the blood vessel. Arrows show cellular junctions between endothelial cells. Scale bars; 150 μm for (A–C), 20 μm for (D–F), and 10 μm for (G–I).

Acknowledgments

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