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FIGURE 3 Expression and distribution of gnaq mRNA during embryonic development and Gαq in section of hypothalamus. (A) Relative expression of gnaq mRNA in the embryonic development stage; Error bars indicate mean ± SEM, n = 3; one-way ANOVA was used for statistical analysis (p < 0.05). (B) whole-mount in situ hybridizations of gnaq; stage: 64-cell, 30%-epiboly, 70%-epiboly, 16-somite, 21-somite, 24 h, 48 h, 72 h, and 96 h. Scale bar: 1000 μm (n = 10); (C) immunohistochemical analysis of Gαq in hypothalamus; SC: secretory cell; DIL: diffuse nucleus of the inferior hypothalamic lobe; ATN: anterior tuberal nucleus; Hc: caudal zone of periventricular hypothalamus; Hv: ventral zone of periventricular hypothalamus; Hd: dorsal zone of periventricular hypothalamus; Cpop: postoptic commissure; CM: corpus mammilare: LR: lateral recess; scale bar: 500 μm; (D) negative control; SC: secretory cell; scale bar: 500 μm.