ZFIN Image: Zadra et al., 2022, Fig. 5

Image

Figure Caption

Fig. 5 a Immunofluorescence images of metaphase spindles in control and siTTLL11 hTERT-RPE1 untransformed cells, showing the PolyE signal (green), tubulin (red) and DNA (blue). Scale bars, 10 μm. b Immunofluorescence images of metaphase spindles in a panel of cancer cell lines as indicated. The PolyE signal (green), tubulin (red), and DNA (blue) are shown. Scale bars, 10 μm. c Quantification of the polyE signal normalized to the total tubulin signal in spindles from hTERT-RPE1 and cancer cells shown in (b). n (RPE1) = 15 cells, n (RPE1 siTTLL11) = 20 cells, n (HT-29) = 15 cells, n (HCT-116) = 41 cells, n (MDA-MD-231) = 10 cells, n (MDA-MD-468) = 11 cells and n (U2O2) = 17 cells. Data are presented as mean values +/− SD. Source data are provided as a Source Data file.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.