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Fig. 6 PRO-INV heterotypic CTC clusters exist in the blood of melanoma patients. a. Schematic of experimental design. b. (left) IF staining for TFAP2A and SOX9 (composite image) and (right) nuclear quantification in a PRO-INV heterotypic melanoma CTC cluster. Scale bar is 5μm. c-d. Classification of human melanoma CTC clusters based on quantification of nuclear TFAP2A and SOX9 staining as (c) homotypic (PRO-only or INV-only) vs. PRO-INV heterotypic, and (d) PRO, PRO-INV, and INV. For c-d n=32 clusters from 4 patients. 5 additional patients were analyzed with no CTC clusters identified. For d each stacked box represents one CTC cluster. See also Figure S6 and Table S7.
Reprinted from Developmental Cell, 56(20), Campbell, N.R., Rao, A., Hunter, M.V., Sznurkowska, M.K., Briker, L., Zhang, M., Baron, M., Heilmann, S., Deforet, M., Kenny, C., Ferretti, L.P., Huang, T.H., Perlee, S., Garg, M., Nsengimana, J., Saini, M., Montal, E., Tagore, M., Newton-Bishop, J., Middleton, M.R., Corrie, P., Adams, D.J., Rabbie, R., Aceto, N., Levesque, M.P., Cornell, R.A., Yanai, I., Xavier, J.B., White, R.M., Cooperation between melanoma cell states promotes metastasis through heterotypic cluster formation, 2808-2825.e10, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell