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Fig. 3
Sonic Hedgehog signaling is not required for laminin-111 protein re-expression in 3.5 dpf spns1−/− larvae. (A–D1) Anterior left, dorsal top, side-mounted 3.5 dpf WT and spns1−/− larvae stained with phalloidin (white) and laminin-111 (cyan) post vehicle control or post 50 μM cyclopamine treatment. (A,A1,C,C1) Phalloidin and laminin-111 staining after EtOH vehicle control treatment in (A,A1) WT and (C,C1) spns1−/− larvae. (A–A1) White arrows point to MTJ in WT larvae. Note absence of laminin-111 protein in WT embryos and presence of laminin-111 protein in spns1−/− larvae. (B,B1,D,D1) Phalloidin and laminin-111 staining after cyclopamine treatment in WT and spns1−/− larvae. White arrowheads point to laminin-111 staining at the MTJ. (E,F) Relative fluorescent intensity of laminin-111 staining in (E) WT and (F) spns1−/− larvae after ethanol or cyclopamine exposure. **** p < 0.0001. Scale bar 50 µm.