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Figure 1 Conservation of BCL6A and related sequences. (A) Synteny analysis of BCL6-related genes. Arrangement of the gene neighborhood surrounding BCL6-related gene loci from human (Homo sapiens, hs), mouse (Mus musculus, mm), zebrafish (Danio rerio, dr) and torafugu (Takifugu rubripes, tr). The BCL6-related genes are in black, neighboring genes conserved between mammals and fish in green, between mammals in blue and between fish in red, with all other genes in grey. (B) Phylogenetic analysis of BCL6-related proteins. The amino acid sequences of fruit-fly Ken and Barbie (Ken) was aligned with the BCL6A and related sequences of human (hs), mouse (mm), zebrafish (dr) and torafugu (tr), and the MYNN-related sequences from human, mouse and torafugu using Clustal W. This was used to construct a phylogenetic tree using the Neighbor-Joining method with 1000 replicates, with bootstrapping values shown. (C) Conserved domains in BCL6A proteins. Human BCL6A and zebrafish Bcl6aa were aligned using Clustal X software, with specific domains highlighted (BTB/POZ in pink, PEST in yellow, zinc fingers in green). Conserved residues between the two sequences are indicated (identical *, highly similar: similar.). (D) Conserved BCL6A gene structure. Schematic diagram of human BCL6A and zebrafish bcl6aa loci, with exons shown as boxes and introns as lines. Regions corresponding to the promoter (grey) or those encoding the BTB/POZ (pink), PEST (yellow) and zinc finger (green and numbered) domains are indicated.