Fig 2

Fig 2 cd9b homozygous mutants have the same number of PGC per embryo at 32 hpf and migration to the gonadal ridge is not affected in cd9b^pg15 mutants at 30 hpf.

(A) There is no significant difference in the number of PGCs in WT and cd9b mutant embryos at 32 hpf. PGCs were visualised using a vasa ISH on 32 hpf embryos and analysed using an ANOVA with Dunnett’s multiple comparisons test on n = minimum 10 embryos per genotype. (B) vasa ISH on 30 hpf WT and cd9b^pg15 embryos. PGC migration efficiency was analysed by looking at the distance between the most anterior and posterior PGC, which should have reached the gonadal ridge by 30 hpf. No significant difference was seen between WT and cd9b mutants as shown by a Mann-Whitney U test. n = at least 13 individual embryos. Data from a single experiment.