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Figure 4
Analysis of mitochondrial respiration in 5 dpf wfs1KO zebrafish larvae
(A) Oxygen consumption rate (OCR) profiles of wfs1abWT and wfs1abKO zebrafish larvae at 5 dpf during the assay and calculated, (B) basal respiration rate, (C) ATP production-related OCR, (D) maximal respiration, (E) proton leak, and (F) non-mitochondrial respiration. Data are mean from triplicates and show mean ± SEM from n = 28 animals per genotype. Olig, oligomycin; FCCP, cyanide-p-trifluoromethoxyphenylhydrazone; Rot/AA, rotenone + antimycin A. ∗p < 0.05, ∗∗p < 0.01 versus wfs1abWT, unpaired t test.
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Full text @ Mol Ther Methods Clin Dev