Fig. 8

Fig. 8

DNA damage response is compromised in zebrafish embryos. (A) Representative western blot showing protein expression or phosphorylation of H2AX ser139 upon the indicated treatment in wildtype, smarcad1a^{sa1299−/−}, and smarcad1a^p403−/− 1dpf zebrafish embryos. For irradiated samples (IR+), samples were harvested at the indicated hour post 15 Gy IR. (B) Quantification of protein expression via western blotting from panel A. For each biological replicate, values were normalized to the value for “IR−” to calculate the fold change in phosphorylation of H2AX upon the indicated treatment. Bars in (B) are the mean of three independent experiments. Statistical analysis comparing experimental to the control (“IR−”) was performed using Welch’s t test (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; NS p > 0.05)