Figure 5

Col2a1a MO knockdown differentially affected Sox10 and Foxd3 expression and ocular neural crest cell migration during early eye development. Lateral live imaging at 24, 36 and 48 hpf showing the effects of Col2a1a knockdown on GFP reporter expression in Tg (foxd3::EGFP) and Tg(sox10::EGFP) transgenic zebrafish embryos injected at the single-cell stage with a lissamine-tagged antisense prMO targeting col2a1a. (A–F) uninjected, (A’–F’) standard control (std ctrl) MO-injected, (A”–F”) col2a1a promoter MO (prMO)-injected. Foxd3-positive cell migration (bold arrows, (F”)) was not affected after MO injection compared with that in std ctrl MO-injected (D”) and uninjected embryos (B”). Sox10-positive cell migration (E”) was markedly disrupted in response to Col2a1a MO knockdown, as these cells were retained in the anterior segment (asterisk) and periocular mesenchyme (single arrows) in contrast to the cells in their uninjected (F) and std ctrl-injected counterparts (F’), which showed few Sox10-positive cells in the anterior segment at 48 hpf. The solid and dashed circles highlight the lens and retinal piment epithelium, respectively, in the zebrafish eyes. d, dorsal; v, ventral; p, posterior; a, anterior.