Fig. 2.

Lack of evidence for modulation of pth2 expression by mechanosensory inputs from inner ear hair cells. (A) Location of pth2-expressing cells in the thalamic region of a larval zebrafish. (B-F) Representative images of mRNA ISH showing pth2-positive cells in socially reared larvae at 4 dpf: (B) WT (n=14); (C) lhfpl5b^vo35 (n=18); (D) tomt^tk256c (n=13); (E) pcdh15a^th263b (n=13), and (F) cacna1da^tc323d (n=18). Scale bar: 50 µm, applies to all images. (G) Boxplots of qPCR results showing pth2 expression in socially reared mutants and siblings at 4 dpf. Ten larval heads were pooled per condition to make one biological replicate, and three biological replicates were used to generate the data. Two technical replicates of the qPCR experiment were conducted per biological replicate and averaged. One-way ANOVA to assess significance; ns, not significant.