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Figure 4
Col11a1a knockdown disrupts the organization of bone forming cells that line the Meckel’s cartilage. Ventral view of sp7:EGFP positive cells in 5 dpf control zebrafish (A,C) and morphant (B,D). sp7:EGFP expressing cells line the cartilage template in the control zebrafish (C). sp7:EGFP expressing cells form small condensations at the mediolateral cartilage but fail to occupy the location of future mineralization immediately adjacent to the Meckel’s cartilage as observed in controls (D); Scale bar 20 μm.
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