Figure 1
Figure 1
(A, B) Lateral views of 24 hpf WT, standard MO-injected, ndrg1a MO-injected and ndrg1a-/- mutant embryos labeled using (A) wholemount in situ hybridization to detect the mRNA distribution of slc20a1a, slc12a3, atp1a1a, and kcnj1a.1 (n=3–4 experiments with an average of 36 embryos per riboprobe) and (B) immunolabeling with anti-ATP1A1A or anti-GFP in Tg[enpep:GFP] transgenic line (n=3–4 experiments with an average of 40 embryos per antibody). Annotations: arrows point to signal in the pronephric duct; arrowheads: ionocytes. Scale bars 300 μm.
Ndrg1a is not required for the differentiation of the pronephric duct and ionocytes.