ZFIN Image: Yong et al., 2021, Fig. 3

Image

Figure Caption

Fig. 3

The FERM domains SNX27 and SNX17 recognize the DLF and NPxY/NxxY motifs, respectively. (A) GST-SNX1^68-90, GST-SNX1⁶⁸^-90DLF, GST-VLDLR, and GST pull-down of purified His₆-SNX27-FERM. Shown is a Coomassie Blue–stained SDS/PAGE gel of purified protein (Left) and bound samples (Right). * indicates the retained His₆-SNX27-FERM (Left) or MBP-SNX17-FERM (Right). (B) The GST-fusion proteins used in A were incubated with purified MBP-SNX17-FERM. Shown is a Coomassie Blue–stained SDS/PAGE gel of purified protein (Left) and bound samples (Right). (C and D) ITC of P-selectin, LRP1, SNX1^68-90, and SNX1^68-90 DLF titrated into MBP-SNX27-FERM (C) or SNX17 FL (D) in a buffer containing 50 mM Tris-Base pH 8.0 or 100 mM NaCl at 16 °C, respectively. Raw data are listed at the top, and integrated normalized data are listed at the bottom. Colors indicate the peptides used in the assay. Experiments were performed in triplicate, and one representative experiment is presented.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA