Fig. 2

Representative 1 h long LFP traces with PTZ treatment: a TL (b) DKO, (c) panx1a^−/−, and (d) panx1b^−/−. TL and panx1b^−/− traces showed typical seizure-like events. Single events were highlighted by red dotted lines and magnified. Blue dotted lines highlight a lack of seizure-like activity in DKO and panx1a^−/− larvae. Spectrograms below the traces showed low and high-frequency power increases during seizure-like events for TL and panx1b^−/−. Scale bar: A value of 0 (blue) is low, and a value of 50 (red) is high. e–h Power spectral density (PSD, mV²/Hz) measured across frequencies revealed no significant differences in baseline (dotted lines) frequencies for all genotypes (P = 0.69; Kruskal–Wallis test). e TL, gray; f DKO, light blue; g panx1a^−/−, dark blue; h panx1b^−/−, magenta. The power was significantly increased in the delta band (1–4 Hz) after PTZ treatment (solid line) for all genotypes except panx1a^−/− (TL: P = 0.02, n = 7; DKO: P = 5.0 × 10⁻⁴, n = 12; panx1a^−/−: P = 0.6, n = 8; panx1b^−/−: P = 3.9 × 10⁻³, n = 9; Wilcoxon signed-rank test). The PSD was plotted as an average across traces with the shaded regions indicating ± s.e.m. Changes in delta power were quantified from the areas under the curves (AUC; highlighted in yellow) of the power spectrum. The changes in AUCs are represented as bar graphs inserted to the right of the power spectra. TL showed the greatest change in delta, followed by panx1b^−/− (P = 1.0), and DKO (P = 0.4). Panx1a^−/− exhibited insignificant changes (P = 2.3 × 10⁻³; Dunn’s multiple comparisons) and maintained delta power throughout recordings. N = number of larvae. Scale bars: top = 0.5 mV by 250 s and bottom = 0.2 mV by 1 s. ***P < 0.001, ****P < 0.0001, gray star - P = 2.3 × 10⁻³ compared to TL with PTZ.