Saccular otoliths are not tethered correctly in the homozygous ext2 mutant ear at 5 dpf. (A–C”’) Live DIC images of embryos at 5 dpf, before (left-hand column) and after (right-hand column) tapping. In phenotypically wild-type sibling embryos (A,A’), saccular otoliths (green arrowheads) remain in place after tapping (in this example, one of the utricular otoliths in (A’) has become slightly displaced). In ext2 mutant embryos (B–D’), the saccular otolith in one or both ears becomes displaced (magenta arrowheads show new position after tapping). (A–C’) are dorsal views with anterior to the left; (C”,C”’) are lateral views of the embryo shown in (C,C’). Note also the swollen ear morphology in the ext2 mutants [(B,C), black arrowheads]. In all panels, anterior is to the left. Scale bar in (A), 100 μm (applies to all panels).
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