ZFIN Image: Zhang et al., 2022, Fig. 6

Image

Figure Caption

Fig. 6

a A schematic representation of GNPTAB processing by S1P. b The processing of the endogenously tagged GNPTAB in TMEM251 KO and TMEM251 overexpression (OE) cells. c Quantification of the GNPTAB processing efficiency in b. Mean of 3 independent replicates is shown. Error bars represent standard deviation. ****p ≤ 0.0001. d A schematic representation of SREBP2 processing by S1P and S2P. e The processing of SREBP2 in HEK293T WT and TMEM251 KO cells. f Quantification of the SREBP2 processing efficiency in e. Mean of 3 independent replicates is shown. Error bars represent standard deviation. **p ≤ 0.01, ***p ≤ 0.001. g A schematic representation of ATF6 processing by S1P and S2P. h ATF6 processing in HEK293T WT and TMEM251 KO cells after 1 h of CHX and DTT treatment. i Quantification of the ATF6 processing efficiency in (h). Mean of 3 independent replicates is shown. Error bars represent standard deviation. **p ≤ 0.01. See source data file for exact P values. j, k Reciprocal IP (n = 2 independent replicates) showing interactions between GNPTAB and TMEM251. l, m Reciprocal IP (n = 2 independent replicates) showing interactions between S1P (S414A) and TMEM251.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.