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Fig. 6
Figure 6. Fluorescent immunohistochemistry expression analysis of myocilin and mCherry in the lens and retina of old (two-year-old) myoc transgenic zebrafish. A chicken anti-myocilin primary antibody was used to detected myocilin (green signal). Control wild type lens (A) and retina (B). Representative head tissue sections in panels (C,D) indicate the regions analyzed by immunohistochemistry in transgenic zebrafish with bilateral (C) or unilateral (D) ocular alterations. Lens (E,F) and retina (G–J) tissue sections. Scale bars: 50 μm. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; LC: lens capsule; LEP: lens epithelium; OA: ocular alterations; OPL: outer plexiform layer; ONL; outer nuclear layer; PHL: photoreceptor layer; +/+: wild type; Tg/+: transgenic.
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