Fig. 7
Relative expression of fos genes in human BRAFV600E oncogene driven melanoma in zebrafish. mRNA expression levels of zebrafish fos genes were determined in normal wildtype (WT) skin (A) and melanoma tumour from BRAFV600E; tp53−/− zebrafish line (B), with the graph showing the expression relative to the normal skin (fold change) (C). The relative mRNA levels were normalised to actb and ddct values determined by comparison with expression in the normal wildtype skin. Error bars indicate the standard error of mean for five biological replicates with statistical significance indicated (*** p < 0.001, ns not significant). The white arrow indicates the tumour.