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Fig. 7
Figure 7. Corneal epithelium and retinal ganglion cell apoptosis in old (two-year-old) myoc transgenic zebrafish. Apoptosis was assessed using terminal dUTP nick-end labelling (TUNEL) of fragmented DNA. (A) Wild-type retina and (B) cornea. (C,D) Representative transgenic head tissue sections indicate the regions analyzed by immunohistochemistry. (E–H) transgenic cornea. (I–L) Transgenic retina. Scale bar in (A,I–L): 25 μm. Scale bar in (B,E–H): 50 μm. (M) Quantification of TUNEL positive cells. Four microscopic fields per eye were analyzed (n = four eyes). ***: p < 0.001, Student’s t-test. White arrowheads: TUNEL-positive cells. Autofl.: tissue autofluorescence used for image contrast and anatomical reference. CEP: corneal epithelium; CST: corneal stroma; CEN: corneal endothelium; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OA: ocular alterations; OPL: outer plexiform layer; ONL; outer nuclear layer; PHL: photoreceptor layer; Tg/+: transgenic; +/+: wild type. The images are representative of the results observed in two fishes of each genotype.
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