Fig. 13

Figure 13. Proliferation of retinal ganglion cells in adult (13-month-old) myocilin transgenic zebrafish. (A,B) An anti-Ki-67 primary antibody was used to detect proliferating cells. Digital magnification of the positive cells indicted with white dotted boxes are shown in the inserts. (C) The negative control consisted of tissue sections incubated only with the secondary antibody. (D,E) Black rectangles in two representative eye sections indicate the location of microscopic fields shown in the different panels. (F) Quantification of Ki-67-positive cells. Four microscopic fields per eye were analyzed (n = four eyes). ***: p < 0.001, Student’s t-test. Scale bars: 50 μm. Arrowheads: Ki-67-positive cells. Scale bars: 50 μm. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PHL: photoreceptor layer. The images are representative of the results observed in two fishes of each genotype.