Fig. 2

scd5-deficient zebrafish showed an apple-shaped body with visceral fat deposits and short spines

(A) Diagram of target site on exon three of zebrafish scd5 genomic DNA, representative sequencing results of scd5^+/+ and scd5^−/− at six dpf larvae, red letter indicates N20 sequence, blue letter indicates NGG.

(B) scd5^−/− zebrafish exhibited differences in body length and body shape at around 30 dpf compared to scd5^+/+ (n = 20).

(C) Phenotype, the 3D reconstruction and section view of micro-CT scans on 4mpf scd5^−/− zebrafish and their wild-type siblings. Black dotted lines indicate sectional position, red dotted line indicates body length, and yellow dotted curve indicates the outline of zebrafish. Blue area in section view indicates visceral adipose tissue. White scale bar = 5mm, Black scale bar = 2mm. D-H. 4mpf scd5^−/− had significant decrease in body

(D) and spine (H) length, significant increases in BMI

(E), zWHtR (F) and VAT (G) (n = 10).

The data are represented as mean ± SD ∗∗∗p < 0.001 represents statistical significance compared with wild-type controls (unpaired t test).