|
Fig. 2
Image
Figure Caption
Fig. 2. npas4l reporter–expressing cells are observed in pronephron tubules and skeletal muscle in npas4l mutants.
(A) npas4l reporter expression in ventral cells of 24 hpf npas4l+/− (left) and npas4l−/− (right) embryos. The main axial vessels (DA and PCV) are visible in npas4l+/− embryos, while only the more ventrally located cells are detected in npas4l−/− embryos (arrowheads). (B) Transverse sections of 20 hpf npas4l+/− (left) and npas4l−/− (right) embryos. The endothelial progenitors (outlined by yellow dotted lines) in npas4l mutants fail to reach the midline unlike those in wild-type siblings; they also remain round. The round ventrolateral npas4l reporter–expressing cells in npas4l−/− embryos also express Pax2a and are part of the pronephron tubules. (C to E) Numbers of npas4l reporter/Pax2a double-positive cells (C), all Pax2a-positive cells (D), and npas4l reporter–expressing skeletal muscle cells (E) per field of view (319.45-μm-long area over the yolk extension). Cells were counted in three-dimensional (3D) confocal lateral views of npas4l+/+, npas4l+/−, and npas4l−/− embryos at 24 hpf. Data are represented as individual data points, median, interquartile range, and extremes excluding outliers. P values were calculated by Poisson regression and adjusted to counteract the multiple testing problem. Data represent a subset of the data shown in Fig. 6 (B to D). DA, dorsal aorta; PCV, posterior cardinal vein. Scale bars, 50 μm (A) and 10 μm (B).
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Sci Adv