Fig. 2

Fig. 2

a The quantification analysis of mRNA levels of key genes involved in the NOD1-RIPK2 signaling pathway in the WT, CD44a^−/−-14del and CD44a^−/−-10IS zebrafish larvae, as determined by qRT-PCR. Quantification data represent the expression level of genes compared with those in the WT zebrafish. b Zebrafish larvae from the WT and NOD1^−/− mutants microinjected with p3×FLAG or CD44a_tv1-FLAG were collected at the indicated post-infection time points, and homogenates were made for CFU counts. c Zebrafish larvae from the WT and NOD1^−/− mutants microinjected with p3×FLAG or CD44a_tv2-FLAG were collected at the indicated post-infection time points, and homogenates were made for CFU counts. d Zebrafish larvae from the WT and CD44a^−/−-14del mutants microinjected with p3×FLAG or NOD1-FLAG were collected at the indicated post-infection time points, and homogenates were made for CFU counts. For a–d, the data are presented as means ± SD (n = 3). *p < 0.05, **p < 0.01; ns not significant.