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Fig. 5
Effect of NYT on BDNF/CREB in NPY-KO zebrafish. The mRNA levels of bdnf and creb1 were estimated by real-time PCR using the whole brain of the control—and NYT-fed NPY-KO zebrafish. (A) bdnf. (B) creb1. The gene expression level of actb was used as an internal reference. Each gene expression level was relative to that in the control-fed NPY-KO zebrafish. n = 5. The protein levels of (C,D) p-CREB and (C,E) CREB were analyzed using immunoblotting with whole-brain lysates of control—and NYT-fed NPY-KO zebrafish. The loading control used was (D) total CREB and (E) β-actin. n = 4. Results are shown as mean ± standard error of the mean. n.s., not significant.