ZFIN Image: Song et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Specification defects of the cardiac and pharyngeal mesoderm upon Gata5/6 loss.

RNA ISH against atrial/pectoral fin progenitor marker nr2f1a (A and B), SHF marker fgf8a (C), posterior pharyngeal arch progenitor marker aldh1a2 (D), and pharyngeal arch one progenitor marker irx1b (E) in WT, Gata5/6 KD embryos, and gata5/6 mutants at 13 hpf. For each gene, quantification and quantification schematics are shown on the right side. Only the lateral staining (colored blue in the schematics) was quantified. For all staining except the anterior expression of nr2f1a, both expression intensity and expression domain sizes were quantified. Since the anterior lateral expression of nr2f1a was not detectable in Gata5/6 KD embryos, we applied the same regions of interest (ROIs) that we used in WT to measure the intensity. Arrowheads in (A), (B), and (D) indicate the lateral domains that show differential expression between WT and Gata5/6-deficient embryos. Stars in (C) denote the unaffected anterior lateral expression of fgf8a in Gata5/6 KD embryos and gata5/6 mutants. Dorsal views are shown. t test was used to determine statistical significance. ***P < 0.001; **P < 0.01; not significant (n.s.), P > 0.05. Scale bars, 100 μm. A.U., arbitrary units.

Acknowledgments

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