ZFIN Image: Sidoli et al., 2022, Fig. 4

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Fig. 4

Figure 4. (A) Confocal images of cAMP (green) and Shh reporter (magenta) signals in control fish and double mutants forgpr161a (st129 allele) and MZgpr161b (st128 allele). MZ = maternal zygotic. Control fish include gpr161a^st129/+, gpr161b^128/+ and gpr161a^st129/129, gpr161b^128/+. (B) Graphs show GFP fluorescence intensity measured in the somite area corresponding to the muscle pioneers in Tg(gli:mCherry-NLS); Tg(ubi:DDcAMP) (control) and in Tg(gli:mCherry-NLS); Tg(ubi:DDcAMP); gpr161a^st129/129; MZgpr161b^128/128 (gpr161 mut) embryos. Error bars indicate SD: ****p < 0,0001 by t-test. (C) Mean of the pixel values in each image acquired in panel A plotted as a function of their frequency. gpr161 double mutant fish display darker values compared to the controls. n = 4–5 animals per genotype. (D) Magnification of somites from 24 hpf Tg(gli:mCherry-NLS) control and Tg(gli:mCherry-NLS); gpr161a^st129/129; MZgpr161b^128/128 embryos injected at one-cell stage with Arl13b-DDcAMP (cyan) and Arl13b-mApple (red cilia). (E) Ratio of ciliary GFP/ciliary mAPPLE in over 20 cilia per animal in Tg(gli:mCherry-NLS) (control) and Tg(gli:mCherry-NLS); gpr161a^st129/129; MZgpr161b^128/128 (gpr161 mut) embryos.

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Acknowledgments

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