ZFIN Image: Agarwala et al., 2022, Fig. 4

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Figure Caption

Fig. 4

3D alignment of light sheet and serial block-face scanning electron microscopy (SBEM) datasets localizes a single rare hematopoietic stem and progenitor cell (HSPC) across multiple imaging modalities.

(A) Single Z plane from light sheet imaging of drl:APEX2-mCherry⁺ transgenic larva showing the lodged mCherry⁺ HSPC (white arrowhead). (B) Global alignment of 3D rendered models generated from light sheet and SBEM datasets using Imaris software. (C) Orthogonal views of the white boxed region within B shows a 3D view of the alignment between light sheet and SBEM datasets. White arrowhead points to the single lodged HSPC in the aligned light sheet and SBEM datasets. (C’) APEX2⁺ HSPC in SBEM data. (C’’) mCherry⁺ HSPC in light sheet data. Green: Injected Oregon Green dextran dye marking vessels. Magenta: Runx:mCherry⁺ HSPCs and autofluorescence in gut. (D) Detail of the alignment shows mCherry⁺ HSPC and APEX2⁺ HSPC are <5 µm apart (dotted white line). Abbreviations: ISVs, intersegmental vessels; D, dorsal; V, ventral; A, anterior; P, posterior.

Acknowledgments

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