ZFIN Image: Jeong et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Impaired NMJs can be restored by inhibiting HDAC6.

(A) Lateral view images after staining with anti-SV2 and α-BTX of a whole-mounted zebrafish injected with control, gars MOs, or treated with tubastatin A (TubA). The merged images are magnified from the images in the rectangular regions. Scale bars = 50 μm. (B) Comparison of fluorescence intensity (green, presynapse; red, postsynapse) among MO-injected and TubA-treated zebrafish embryos. (C-E) Comparison of presynapse (C), postsynapse (D), and NMJ (E) signal ratios within the ROI among zebrafish embryos injected with control MOs (n = 24), gars MOs (n = 17), and gars MOs with TubA (n = 21). Statistical significance was assessed using one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001. ns, non-significant. (F and G) Comparison of NMJ innervation in the presynaptic (F) and postsynaptic (G) areas among zebrafish embryos injected with control MOs (n = 23), gars MOs (n = 29), and gars MOs with a TubA treatment (n = 36). Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test. ***P < 0.001. ns, non-significant.

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Acknowledgments

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