ZFIN Image: Osorio-Méndez et al., 2022, Fig. 3

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Fig. 3

scn8ab Is dispensable during early development. (A) Number of zebrafish with scn8ab^+/+, scn8ab^+/Δ, and scn8ab^Δ/Δ genotypes scored at 5 dpf (Left) and at 3 mpf (Right). The observed genotypic ratio matched the expected ratio in 5 dpf larvae from scn8ab^Δ/+ in-crosses, but no scn8ab^Δ homozygotes recovered at adult stages (>3 mpf). (B) Representative whole-mount images of control (heterozygous sibling) and temca at 3 mpf. (C and D) Quantification of body weight (C) and body length (D) at 3 mpf (n = 41 and 53 for scn8ab^temca/+ and scn8ab^temca/^Δ, respectively). (E) Control (heterozygotes) and temca 6 dpf larvae swimming tracks at 34 °C. (F) Quantification of swimming behavior of 6 dpf control and temca larvae (n = 16 and 20 for control and temca, respectively). Two-way ANOVA, temperature [F(1,33) = 0.01992, P = 0.889]; genotype [F(1,33) = 0.005127, P = 0.943]; genotype × temperature interaction [F(1,33) = 4.119, P = 0.051]. (G) Representative images of control (heterozygotes) and temca regenerated fin folds 3 dpa at 33 °C. (H) Quantification of 3 dpa larvae fin fold–regenerate lengths at 33 °C (n = 13 and 17 for control and temca, respectively). Scale bars, 1 cm in B, 200 µm in G. (C, D, and H) Data are presented as mean ± SD by Student’s unpaired two-tailed t test.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA