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Fig. 4
rab28 knockout zebrafish have reduced levels of retinal pigment epithelium (RPE) phagosomes containing OS, which can be rescued using cone-specific expression of eGFP; Rab28 (A) Time course highlighting sample collection time (red x) in relation to RPE phagosome numbers. (B) Scatter plots of phagosome/μm of RPE in rab28 sibling, KO, and eGFP-Rab28 variants in the KO background. rab28 KO fish have ~55% reduction in RPE phagosomes containing engulfed OS material in comparison with sibling controls. rab28 KO expressing Tg(eGFP:Rab28WT,QL,TN) rescue this phenotype and do not show reduced numbers of RPE phagosomes containing engulfed OS shed material. Statistical analysis was performed using a one-way anova, followed by a Bonferroni multiple comparison test. Error bars show SEM. N = 3 animals per genotype. (C–G) Representative TEM images of rab28 sibling control, KO and KO expressing Tg(eGFP:Rab28WT,TN, QL) retinae at 15 days post fertilization, with phagosomes indicated (pink arrows). Scale bar; 2 μm. RPE of >350 μm was analyzed per animal.
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Full text @ FASEB J.