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Fig. 3
rab28 knockout zebrafish have reduced levels of retinal pigment epithelium (RPE) phagosomes, at 2 known outer segment phagocytosis peaks. (A) Time course highlighting sample collection time (red x) in relation to phagosome level fluctuation. (B) Trend line demonstrating the level of RPE phagosomes present in rab28 KO (red) compared with sibling controls (blue) at defined time points throughout the day. (C–J) Representative TEM images of rab28 sibling controls and KO retinae at 15 days post fertilization, with phagosomes indicated (pink arrows). Scale bar; 2 μm, (K–N) Scatter plots of phagosome/μm of RPE in rab28 sibling and KO retinas at distinct time points. rab28 KO fish have ~55% reduction in OS engulfed RPE phagosomes in comparison with sibling controls at ZT 4 and ZT 17. Statistical analysis carried out using an unpaired student's t test for each distinct time point. RPE of >350 μm was analyzed per animal. N = 6 retinae per genotype at ZT 4 and ZT 17, N = 2 retinae per genotype at ZT 0 and ZT 12
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